CRYOPRESERVED SEMEN OF <i>Piaractus orinoquensis</i> (SERRASALMIDAE): POST-THAW STORAGE TIMES AND ACTIVATING SOLUTIONS

Piaractus orinoquensis is a native species from the Colombian Orinoquia and several studies have contributed to standardization of protocols for seminal cryopreservation this species. The main goal study was evaluate sperm motility, duration motility (DM), pH, membrane integrity (SMI) fertility semen cryopreserved seven years subjected different post-thaw storage times (PST). Semen five males (3.04 ± 0.3 kg) diluted 1:4 (semen:diluent) with 10 % dimethylsulfoxide, 5.5 glucose 12 egg yolk in 4 mL macrotubes frozen liquid nitrogen. Four PST were evaluated: 0, 15, 45 60 min. cells activated 1 NaHCO3 (SB) 0.9 NaCl (SC) determine DM, rate, SMI pH through PST. Significative reduction (p < .05) observed between 0 min both treatments SB SC. had significant after did not vary during rate decreased significantly time affects quality P. orinoquensis, best fertilization results obtained by activating post-thawing SB. long-term (seven years) maintains its capacity values like those fresh sperm.